Microbiology 241 - Biotechnology Lecture

225 Responses | Created by kisskers14 |
  1. 1

    which of the following is a brute force method of DNA insertion?

  2. 2

    Which of the following are theraputic products made by genetic engineering?

  3. 3

    What kind of vector would you use if you wanted to specifically control the amount of gene expression?

  4. 4

    Which of the following is produced downstream from lacO on the pSE420 expression vector?

  5. 5

    Which of these is true regarding the D-Ti vector in the binary vector system of Argobacterium tumefaciens into plants?

  6. 6

    What is the modern method of creating mutant bacteria with desired expression of certain genes?

  7. 7

    How can you tell if a bacterial culture has taken up your pUC19 vector, on to which you have inserted a gene in the polylinker region of the lacZ gene?

  8. 8

    Which of the following is a disadvantage of using E. coli as a cloning host?

  9. 9

    Which of the following is true about the engineering of human insulin hormone?

  10. 10

    The example of lysine overproduction by Brevibacterium flavum mutants is an example of biotechnology. What was the role of AEC (S-aminoethylcysteine) in this pathway?

  11. 11

    Which is NOT an advantage of Saccharomyces cervisiae as a cloning host?

  12. 12

    Transduction is

  13. 13

    Which of the following is not an advantage of Bacillus subtilis as a molecular cloning host?

  14. 14

    How can you tell if a bacterial culture has taken up your UNmodified pUC19 cloning vector?

  15. 15

    What is a problem with expression of human genes in prokaryotes?

  16. 16

    Bioremediation is

  17. 17

    What is the enzyme responsible for producing cDNA by copying information from RNA to DNA?

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